質問編集履歴

1

file.bamをFASTAに変換したものをアップロードしました。

2020/09/19 06:36

投稿

kak
kak

スコア28

test CHANGED
File without changes
test CHANGED
@@ -202,65 +202,7 @@
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  GAATGAGT
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- CTTTGACA
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+
206
-
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- AGAGATCT
208
-
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- GAGTCCTG
210
-
211
- CACACTGA
212
-
213
- GTTACAGG
214
-
215
- GGACCTTT
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-
217
- TAGACTAT
218
-
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- ACTGTTTG
220
-
221
- AAGTGGCT
222
-
223
- TGCTCTCA
224
-
225
- CGGCGTGG
226
-
227
- GTGCATGA
228
-
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- GTCATTAG
230
-
231
- AGCTCCTT
232
-
233
- TCACCCGA
234
-
235
- GTTGCCAC
236
-
237
- CTAATGCG
238
-
239
- AACGAGGT
240
-
241
- AGCCACCA
242
-
243
- TAGTGAAC
244
-
245
- CCAGTCCA
246
-
247
- ACCTCAGC
248
-
249
- GGTGGACT
250
-
251
- CCGGCGTC
252
-
253
- TAACTCCG
254
-
255
- ACACCGTG
256
-
257
- GTAGAACG
258
-
259
- GGATTGAC
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-
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- ACGTATCC
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-
263
- TTCGGAAA
264
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@@ -273,3 +215,217 @@
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  エラーの原因、修正箇所を教えて頂けないでしょうか。
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  お願いします。
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+
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+ <追記>
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+
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+ file.bamは以下のような形です。
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+
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+ BAMファイルの読み方が分からず、
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+
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+ samtools fasta file.bam > file.fa
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+
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+ で変換したものが以下です。
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+
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+ ```ここに言語を入力
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+
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+ >M01400:201:000000000-B57RM:1:1113:26374:10799
232
+
233
+ TAGTCCCAGCTGCTCAGGAGGCTGAAGCAGGAGAATTACTTGAACTGAAGCGGAGGTTGCAGTGAGCTGAGATCACGCCACTGCACCTCCAGCCTGGGTAACAAAGAGAGACA
234
+
235
+ >M01400:201:000000000-B57RM:1:1119:26033:11432
236
+
237
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAGGTATTCA
238
+
239
+ >M01400:201:000000000-B57RM:1:1107:18890:12462
240
+
241
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGTTTTCAA
242
+
243
+ >M01400:201:000000000-B57RM:1:2108:16617:8847
244
+
245
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGCGTA
246
+
247
+ >M01400:201:000000000-B57RM:1:2102:14973:23007
248
+
249
+ GCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGT
250
+
251
+ >M01400:201:000000000-B57RM:1:1119:11253:10536
252
+
253
+ CTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAGAGGT
254
+
255
+ ...
256
+
257
+ >M01400:201:000000000-B57RM:1:2110:20940:24899
258
+
259
+ CTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA
260
+
261
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTT
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+
263
+ >M01400:201:000000000-B57RM:1:1101:5379:9468
264
+
265
+ >M01400:201:000000000-B57RM:1:1112:19959:11614
266
+
267
+ >M01400:201:000000000-B57RM:1:1111:17929:2377
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+
269
+ >M01400:201:000000000-B57RM:1:2114:10378:19188
270
+
271
+ >M01400:201:000000000-B57RM:1:2107:3172:12498
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+
273
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTGCCC
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+
275
+ >M01400:201:000000000-B57RM:1:1109:7422:9436
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+
277
+ >M01400:201:000000000-B57RM:1:1112:25404:3648
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+
279
+ >M01400:201:000000000-B57RM:1:1114:10367:23476
280
+
281
+ CCCTCCCAAAGTTAACTAATCCTCAGAGACTGCAAACAACCTACCTGTGAGCATGCCTTTGATATGCAA
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+
283
+ >M01400:201:000000000-B57RM:1:2105:21791:8292
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+
285
+ CTTCCGATCTCACTTATCTTGACTATAGGAGAGATAAGTG
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+
287
+ >M01400:201:000000000-B57RM:1:2111:6584:14960
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+
289
+ >M01400:201:000000000-B57RM:1:1116:11971:15695
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+
291
+ >M01400:201:000000000-B57RM:1:2107:14103:2153
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+
293
+ >M01400:201:000000000-B57RM:1:2112:6123:8257
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+
295
+ >M01400:201:000000000-B57RM:1:1112:16612:2657
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+
297
+ AGTCATCATCTAATGGAATTGCATGGAATCATCATCAAATGGAATCGAATGGAATCAACATCAAATGGAATCAAATGGAATCATTGAACGGA
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+
299
+ >M01400:201:000000000-B57RM:1:2116:3980:8645
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+
301
+ >M01400:201:000000000-B57RM:1:1108:15797:6718
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+
303
+ ATGGTGCCCTCTTGCTTCTAAAAAGCAAGACTGCTTTGTAATGGCTGCTCT
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+
305
+ >M01400:201:000000000-B57RM:1:1116:7506:23225
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+
307
+ >M01400:201:000000000-B57RM:1:2113:16155:14842
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+
309
+ TGTTCAACTCCCACTTATGAGTGAGAACATGGGGTATTTGGTTTTCTCTTCCTGTGTT
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+
311
+ >M01400:201:000000000-B57RM:1:1104:23739:8369
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+
313
+ GAATTCACTCGGAATAGGAAATCTCAGCCTGATTCTCCCCCAGCATCTCCCTCCCCTATAGAGCAATGCCTGT
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+
315
+ >M01400:201:000000000-B57RM:1:2108:13627:25167
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+
317
+ CTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC
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+
319
+ >M01400:201:000000000-B57RM:1:1117:20846:18468
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+
321
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTCGAA
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+
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+ >M01400:201:000000000-B57RM:1:1119:28174:17064
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+
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+ >M01400:201:000000000-B57RM:1:2105:23616:19481
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+
327
+ AGTGGCCTGGGTCCCATTCTTTCTGAAGTCTCTCCCTCTCCAGCTCTGGACTGCATCGTCGCCCACAGAGGGGATGCTTCTTATCCGGTGG
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+
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+ >M01400:201:000000000-B57RM:1:1102:3493:9712
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+
331
+ ATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATCACCTGAAGTCAGGAGTTTGAGACCAGCTTGACCAACATAGTGA
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+
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+ >M01400:201:000000000-B57RM:1:2116:22487:4781
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+
335
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGTAAGATCGGAAAA
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+
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+ >M01400:201:000000000-B57RM:1:1118:21295:23935
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+
339
+ AGTCCATGGGTTATTCCATTCCATTCAATTAGATAATTCCATTC
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+
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+ >M01400:201:000000000-B57RM:1:1110:20805:19431
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+
343
+ >M01400:201:000000000-B57RM:1:1116:25365:19844
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+
345
+ >M01400:201:000000000-B57RM:1:2105:17405:22459
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+
347
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTAGCGG
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+
349
+ >M01400:201:000000000-B57RM:1:2110:24292:21276
350
+
351
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAG
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+
353
+ >M01400:201:000000000-B57RM:1:1115:11693:3270
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+
355
+ >M01400:201:000000000-B57RM:1:2104:19336:14696
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+
357
+ CTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA
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+
359
+ >M01400:201:000000000-B57RM:1:2102:27456:10532
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+
361
+ ACTAGCCGGGCTTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCA
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+
363
+ >M01400:201:000000000-B57RM:1:2114:7890:12218
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+
365
+ >M01400:201:000000000-B57RM:1:1102:12104:24151
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+
367
+ >M01400:201:000000000-B57RM:1:2111:18205:18888
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+
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+ >M01400:201:000000000-B57RM:1:2107:4141:15987
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+
371
+ >M01400:201:000000000-B57RM:1:1119:13339:17827
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+
373
+ >M01400:201:000000000-B57RM:1:1109:20097:6551
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+
375
+ TCTATTTTAATGCATGGCTTCGGATGTTAAGAATACTTAATGAAAATGTCAAAACTTGGCAATTTTCAGCAGAAACTCA
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+
377
+ >M01400:201:000000000-B57RM:1:2119:24934:23194
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+
379
+ >M01400:201:000000000-B57RM:1:2101:13434:14822
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+
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+ >M01400:201:000000000-B57RM:1:1112:27249:14630
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+
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+ CTGCTCTGGAGGCTGCTGCAGTTGTCTGGGTGAGAGACGGTGGTGGCTTGGACCA
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+
385
+ >M01400:201:000000000-B57RM:1:2102:28197:13960
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+
387
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGA
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+
389
+ >M01400:201:000000000-B57RM:1:2105:29041:15461
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+
391
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGA
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+
393
+ >M01400:201:000000000-B57RM:1:2119:21220:15826
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+
395
+ ATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGA
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+
397
+ ...
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+
399
+ >M01400:201:000000000-B57RM:1:2112:8709:10847
400
+
401
+ >M01400:201:000000000-B57RM:1:2109:15574:3676
402
+
403
+ >M01400:201:000000000-B57RM:1:2113:23952:23774
404
+
405
+ >M01400:201:000000000-B57RM:1:2118:18170:22640
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+
407
+ >M01400:201:000000000-B57RM:1:2108:20065:19990
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+
409
+ AGAAAGAAACATAAAGTAGATGGTGGTTGCCAGGGTTTGGATGGAAGGGGGCATAGGAGTGACTACTAACA
410
+
411
+ >M01400:201:000000000-B57RM:1:1101:10277:16222
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+
413
+ ATTTCGCTCGCTCAGCGGGAAGACCTTGGTCAGCGTGAGGGTCTGTGT
414
+
415
+ >M01400:201:000000000-B57RM:1:1101:10348:17597
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+
417
+ ATTTCGCTCGCTCAGCGGGAAGACCTTGGTCAGCGTGAGGGTCTGTGT
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+
419
+ >M01400:201:000000000-B57RM:1:1101:10673:12347
420
+
421
+ ATTTCGCTCGCTCAGCGGGAAGACCTTGGTCAGCGTGAGGGTCTGTGT
422
+
423
+ >M01400:201:000000000-B57RM:1:1101:10824:16029
424
+
425
+ ATTTCGCTCGCTCAGCGGGAAGACCTTGGTCAGCGTGAGGGTCTGTG
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+
427
+
428
+
429
+ ...
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+
431
+ ```