no python application found, check your startup logs for errors の原因特定方法について

前提・実現したいこと

EC2（ amazon linux 2 ） + nginx + python3.6 + django + uWSGI でデプロイさせるため下記のサイトを参考にしました。

http://sounanda.jp/itskillup/?p=1193

STATIC ROOT :

[root@ip-XXX-XXX-XXX-XXX static]# pwd
/var/www/python-my-app/src/static

発生している問題・エラーメッセージ

no python application found, check your startup logs for errors

nginx.conf

# For more information on configuration, see:
#   * Official English Documentation: http://nginx.org/en/docs/
#   * Official Russian Documentation: http://nginx.org/ru/docs/

user nginx;
worker_processes auto;
error_log /var/log/nginx/error.log;
pid /run/nginx.pid;

# Load dynamic modules. See /usr/share/doc/nginx/README.dynamic.
include /usr/share/nginx/modules/*.conf;

events {
    worker_connections 1024;
}

http {
    log_format  main  '$remote_addr - $remote_user [$time_local] "$request" '
                      '$status $body_bytes_sent "$http_referer" '
                      '"$http_user_agent" "$http_x_forwarded_for"';

    access_log  /var/log/nginx/access.log  main;

    sendfile            on;
    tcp_nopush          on;
    tcp_nodelay         on;
    keepalive_timeout   65;
    types_hash_max_size 4096;

    include             /etc/nginx/mime.types;
    default_type        application/octet-stream;

    # Load modular configuration files from the /etc/nginx/conf.d directory.
    # See http://nginx.org/en/docs/ngx_core_module.html#include
    # for more information.
    include /etc/nginx/conf.d/*.conf;
    upstream app_server {
        server 127.0.0.1:8000 fail_timeout=0;
    }
    server {
        #listen       80;
        #listen       [::]:80;
        #server_name  _;
        #root         /usr/share/nginx/html;

        listen 80;
        server_name ec2-XXX-XXX-XXX-XXX.ap-northeast-1.compute.amazonaws.com;
        client_max_body_size 4G;

        # Load configuration files for the default server block.
        include /etc/nginx/default.d/*.conf;

        location / {
            # 以下4行を追加
            proxy_set_header X-Forwarded-For $proxy_add_x_forwarded_for;
            proxy_set_header Host $http_host;
            proxy_redirect off;
            proxy_pass http://app_server;
        }
        error_page 404 /404.html;
            location = /40x.html {
        }

        error_page 500 502 503 504 /50x.html;
            location = /50x.html {
        }
    }